| Sıra |
Tanımı/Adı |
Miktar |
Birim |
Alım Kalemi Şartnamesi |
Genel Şartname |
| 1 |
Acetic acid ReagentPlus®, ?99% 1L, Sigma A6283-1L veya muadili |
1 |
Adet |
|
Teknik_Sartname.doc |
| 2 |
Ammonium persulfate for molecular biology, for electrophoresis, ?98% 25gr, Sigma A3678-25G veya muadili |
1 |
Adet |
|
| 3 |
Anti-HisG Epitope Tag Antibody, 50 µl, 25 rnx, R941-25, Thermo, veya muadili |
2 |
Adet |
|
| 4 |
Blocker™ BSA (10X) in TBS 125ml Thermo 37520 veya muadili |
2 |
Adet |
|
| 5 |
B-PER™ Complete Bacterial Protein Extraction Reagent, 250 ml Thermo 89821 veya muadili |
2 |
Adet |
|
| 6 |
Brilliant (Bromophenol) Blue 25gr, Sigma B0126-25G veya muadili |
1 |
Adet |
|
| 7 |
Certified™ Molecular Biology Agarose 25gr, Biorad 1613100 veya muadili |
2 |
Adet |
|
| 8 |
Champion™ pET100 Directional TOPO® Expression Kit with BL21 Star™ (DE3) One Shot® Chemicall K100-01, Thermo Scientific veya muadili |
1 |
Adet |
|
| 9 |
Chloral hydrate crystallized, ?98.0%, 250 gr Sigma 23100-250G veya muadili |
1 |
Adet |
|
| 10 |
Cleaning Concentrate, 1kg 50X Biorad 1610722 veya muadili |
2 |
Adet |
|
| 11 |
Coomassie Brilliant Blue R-250 Staining Solution, 1L, 1610436, Biorad veya muadili |
1 |
Adet |
|
| 12 |
Dithiothreitol (DTT) 1 g Biorad 1610610 veya muadili |
1 |
Adet |
|
| 13 |
DreamTaq™ Hot Start Green PCR Master Mix, 1000rxn, K9022, Thermo Scientific veya muadili |
2 |
Adet |
|
| 14 |
EDTA, 500 g, ?99% pure Biorad, 1610729veya muadili |
1 |
Adet |
|
| 15 |
Eppendorf tüpü 1,5 ml DNAse RNAse Free 500 adet/paket (her pakette 500 adet bulunan 4 paket) |
4 |
Paket |
|
| 16 |
Eppendorf tüpü 2 ml DNAse RNAse Free 500 adet/paket (her pakette 500 adet bulunan 4 paket) |
4 |
Paket |
|
| 17 |
Ethidium Bromide Solution, 10 ml, Biorad 1610433 veya muadili |
1 |
Adet |
|
| 18 |
Filtreli pipet ucu DNAse RNAse free 10ul 10X96rack (her biri 96 adet pipet ucu içeren 10 racklık 5kutu) |
5 |
Kutu |
|
| 19 |
Filtreli pipet ucu DNAse RNAse free 100ul 10X96rack (her biri 96 adet pipet ucu içeren 10 racklık 5kutu) |
5 |
Kutu |
|
| 20 |
Filtreli pipet ucu DNAse RNAse free 1000ul 10X60rack (her biri 60 adet pipet ucu içeren 10 racklık kutu) |
5 |
Kutu |
|
| 21 |
GelRed 0,5ml Biotium 41003 veya muadili |
2 |
Adet |
|
| 22 |
GeneJET Gel Extraction Kit 250 prep K0692, Thermo veya muadili |
1 |
Adet |
|
| 23 |
GeneJET Plasmid Miniprep Kit 250 rxn Thermo K0503 veya muadili |
1 |
Adet |
|
| 24 |
GeneRuler 100bp plus DNA Ladder Thermo SM0321 veya muadili |
2 |
Adet |
|
| 25 |
Guanidine hydrochloride for molecular biology, ?99%, 100gr, Sigma G3272-100G veya muadili |
1 |
Adet |
|
| 26 |
Halt™ Protease Inhibitor Cocktail (100X) 5ml Thermo 78429 veya muadili, |
2 |
Adet |
|
| 27 |
His Buffer Kit, GE Healthcare, 11-0034-00, veya muadili |
2 |
Adet |
|
| 28 |
HisTrap FF Crude, 5 x 1 ml, GE 11000458 veya muadili |
2 |
Adet |
|
| 29 |
HiTrap Desalting, 5 x 5 ml, GE 17140801 veya muadili |
2 |
Adet |
|
| 30 |
Inclusion Body Solubilization Reagent 100ml, Thermo 78115 veya muadili |
2 |
Adet |
|
| 31 |
IPTG Solution, ready-to-use, 10 x 1.5 mL, R1171 Thermo Sc. veya muadili |
1 |
Adet |
|
| 32 |
Luminaris Color HiGreen qPCR Master Mix, 500 x 20 µl rxns, K0392 Thermo veya muadili |
2 |
Adet |
|
| 33 |
Lysozyme from chicken egg White lyophilized powder, protein ?90 %, ?40,000 units/mg protein, 1gr, Sigma L6876-1G veya muadili |
2 |
Adet |
|
| 34 |
MagicMedia™ E. coli Expression Medium 1lt, K6803, Thermo Sc. veya muadili |
4 |
Adet |
|
| 35 |
Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase 20rxn K1681 Thermo Scientific veya muadili |
1 |
Adet |
|
| 36 |
Microseal® 'A' PCR Plate and PCR Tube Sealing Film 50ad/pkt (bir pakette 50 adet bulunmaktadır) |
1 |
Paket |
|
| 37 |
Nickel(II) sulfate exahydrate ReagentPlus®, powder or crystals, 250gr, Sigma N4882-250G veya muadili |
1 |
Adet |
|
| 38 |
Nitrocellulose Membrane, 0.2 µm, 30 cm x 3 m, 1 rulo, 1620097, Bio-Rad veya muadili |
1 |
Adet |
|
| 39 |
N,N,N',N'-Tetramethylethylenediamine 25ml, Sigma T9281-25ML veya muadili |
1 |
Adet |
|
| 40 |
Novex® Tris-Glycine SDS Running Buffer (10X) 5 L, LC 2675-5, Thermo Sci. veya muadili |
1 |
Adet |
|
| 41 |
Novex™ Tris-Glycine SDS Sample Buffer (2X) 20ml, Thermo LC2676 veya muadili |
2 |
Adet |
|
| 42 |
Novex® Tris-Glycine Transfer Buffer (25X) 500mL LC3675, Thermo Scientific veya muadili |
2 |
Adet |
|
| 43 |
PCR tüpü 0,2 ml DNAse RNAse Free, 1000 adet/paket (her pakette 1000 adet bulunan 5 paket) |
5 |
Paket |
|
| 44 |
Petri Kutusu (P.S) Gama Steril 90 mm, 700adet/kt (kutuda 700 adet bulunan 1 kutu) |
1 |
Kutu |
|
| 45 |
pGEM®-T Easy Vector System 20rxn Promega A1360 veya muadili |
2 |
Adet |
|
| 46 |
Phusion Green High-Fidelity DNA Polymerase (2 U/µL) 500 units F534L Thermo Scientific veya muadili |
2 |
Adet |
|
| 47 |
PlusOne Glycerol 1 Lt, 17-1325-01 GE Healtcare veya muadili |
1 |
Adet |
|
| 48 |
PMSF Protease Inhibitor, 5gr, Thermo 36978 veya muadili |
1 |
Adet |
|
| 49 |
Precision Plus Protein™ Unstained Protein Standards, Strep-tagged recombinant, 1 ml, Biorad #1610363 veya muadili |
2 |
Adet |
|
| 50 |
Precision Plus Protein™ WesternC™ Blotting Standards, value pack, 50 applications, Biorad #1610385 veya muadili |
2 |
Adet |
|
| 51 |
Precision Protein™ StrepTactin-HRP Conjugate, 300 µl #1610380 veya muadili |
1 |
Adet |
|
| 52 |
Primer 0,2 nmol MOPC pürifiye 300bp/set |
1 |
Set |
|
| 53 |
Proteinase K Solution, 5 mL/adet, 4333793, Thermo veya muadili |
2 |
Adet |
|
| 54 |
Qubit™ Assay Tubes 500ad/kutu Q32856, Thermo Sc. veya muadili |
2 |
Adet |
|
| 55 |
Qubit dsDNA BR Assay Kit 100 rxn Q32850, Thermo Sc., veya muadili |
1 |
Adet |
|
| 56 |
Qubit™ Protein Assay Kit 100 rxn Q33211, Thermo Sc., veya muadili |
1 |
Adet |
|
| 57 |
Qubit™ RNA BR Assay Kit 100 rxn Q10210, Thermo Sc., veya muadili |
1 |
Adet |
|
| 58 |
SOC medium 10X10ml 15544034 Thermo veya muadili |
3 |
Adet |
|
| 59 |
Sodium acetate anhydrous, for molecular biology, ?99% 250gr Sigma S2889-250G veya muadili, |
1 |
Adet |
|
| 60 |
Sodium chloride BioXtra, ?99.5% (AT), 250 gr Sigma S7653-250G veya muadili |
1 |
Adet |
|
| 61 |
Sodium dodecyl sulfate L3771-100GR SIGMA veya muadili |
2 |
Adet |
|
| 62 |
Sodium hydroxide BioXtra, ?98%, 500gr Sigma, S8045-500G veya muadili |
1 |
Adet |
|
| 63 |
Sodium phosphate dibasic for molecular biology, ?98.5% (titration), 500 gr Sigma S3264-250G veya muadili, |
1 |
Adet |
|
| 64 |
SuperSignal™ West Pico PLUS Chemiluminescent Substrate, Thermo, 34577 veya muadili |
2 |
Adet |
|
| 65 |
SuperSignal® Western Blot Enhancer 500ml, 46640, Thermo Sc., veya muadili |
1 |
Adet |
|
| 66 |
TC FLASK 25CM2 Cell Culture Treated, 160ad/pk (her pakette 160 adet bulunan 4 kutu) |
4 |
Kutu |
|
| 67 |
TGX Stain-Free™ FastCast™ Acrylamide Kit, 10% BioRad, 1610183TA veya muadili |
2 |
Adet |
|
| 68 |
TRIzol™ Reagent 200ml Thermo 15596018 veya muadili, |
1 |
Adet |
|
| 69 |
Tris Pkg of 1, 500 g, ?99.8% pure, Biorad 1610716 veya muadili |
2 |
Adet |
|
| 70 |
Triton™ X-100 for molecular biology, 50ml Sigma T8787-100ML veya muadili |
1 |
Adet |
|
| 71 |
TWEEN® 20 for molecular biology, viscous liquid Sigma P1379-100ML veya muadili, |
2 |
Adet |
|
| 72 |
Urea 8M, 6X25ml, Sigma U4883-6X25ML veya muadili |
1 |
Adet |
|
| 73 |
Water, nuclease-free 30ml R0582 Thermo veya muadili |
2 |
Adet |
|
| 74 |
1x Tris Buffered Saline (TBS) with 1% Casein 1lt, BioRad 1610782 veya muadili |
2 |
Adet |
|
| 75 |
10x Tris/Glycine Buffer for Western Blots and Native Gels Biorad, 1L, 1610734 veya muadili |
2 |
Adet |
|
| 76 |
2-Mercaptoethanol BioReagent, 99% Sigma M3148-100ML veya muadili |
1 |
Adet |
|
| 77 |
50X TAE buffer 1lt Thermo B49 veya muadili |
2 |
Adet |
|
