| Sıra |
Tanımı/Adı |
Miktar |
Birim |
Alım Kalemi Şartnamesi |
Genel Şartname |
| 1 |
Acetic acid (glacial) 100% 2,5 l, Merck 1018302500 veya muadili |
1 |
Adet |
|
Teknik S¸artname.doc |
| 2 |
Biomax Agarose 100 g, Prona HS-8000 veya muadili |
5 |
Adet |
|
| 3 |
Bromophenol blue 5 gr, Merck 1081220005 veya muadili |
1 |
Adet |
|
| 4 |
Champion™ pET100 Directional TOPO® Expression Kit with BL21 Star™ (DE3) One Shot® Chemically Competent E. coli, Thermo Scientific K10001 veya muadili |
2 |
Adet |
|
| 5 |
Cleaning Concentrate, 1 kg, Biorad 1610722 veya muadili |
2 |
Adet |
|
| 6 |
CloneJET™ PCR Cloning Kit, 40 react, Thermo Scientific MBT-K1232 veya muadili |
7 |
Adet |
|
| 7 |
Coomassie Brilliant Blue R-250 Staining Solution 1 l, Bio-Rad 161-0436 veya muadili |
1 |
Adet |
|
| 8 |
dNTP Mix, 10 mM each, 5x1 ml, Thermo Scientific MBT-R0193 veya muadili |
3 |
Adet |
|
| 9 |
Eppendorf tüpü 1,5 ml DNAse RNAse Free 1000 adet/paket (1000 adetlik paket) |
5 |
Paket |
|
| 10 |
Eppendorf tüpü 2 ml DNAse RNAse Free 1000 adet/paket (1000 adetlik paket) |
5 |
Paket |
|
| 11 |
FastDigest Aval (Eco88I) Unit Size 200 rxns Thermo Scientific MBT- FD0384 veya muadili |
4 |
Adet |
|
| 12 |
FastDigest XbaI Unit Size 750 rxns Thermo Scientific MBT- FD0685 veya muadili |
4 |
Adet |
|
| 13 |
Filtreli Pipet Ucu 100-1000 ul-(10x60) rack DNAse RNAse free/kutu (10x60 racklık kutu) |
10 |
Kutu |
|
| 14 |
Filtreli Pipet Ucu 1-10 ul-(10x96) rack DNAse RNAse free/kutu (10x96 racklık kutu) |
10 |
Kutu |
|
| 15 |
Filtreli Pipet Ucu 1-200 ul-(10x96) rack DNAse RNAse free/kutu (10x96 racklık kutu) |
10 |
Kutu |
|
| 16 |
GeneJET Genomic DNA Purification Kit Unit Size 250 preps Thermo Scientific MBT- K0722 veya muadili |
8 |
Adet |
|
| 17 |
GeneJET Plasmid Miniprep Kit Unit Size 250 preps Thermo Scientific MBT- K0503 veya muadili |
4 |
Adet |
|
| 18 |
GeneRuler 100 bp Plus DNA Ladder, ready-to-use Unit Size 5 x 50 µg Thermo Scientific MBT- SM0324 veya muadili |
4 |
Adet |
|
| 19 |
Glycerol, Molecular Biology Grade, 99%, 1lt, Sigma G5516 veya muadili |
3 |
Adet |
|
| 20 |
Hydrochloric acid, 1l, Merck 1090631000 veya muadili |
1 |
Adet |
|
| 21 |
Hydrogen peroxide 30%, 1 l, Merck 1072981000 veya muadili |
1 |
Adet |
|
| 22 |
IPTG (isopropyl-thio-2- Dgalactopyranoside), 5 g, Qiagen 129921 veya muadili |
1 |
Adet |
|
| 23 |
LB Broth (Lennox) microbial growth medium, Sigma L7275 veya muadili |
5 |
Adet |
|
| 24 |
LB Broth with agar (Lennox) microbial growth medium Sigma L7025 veya muadili |
5 |
Adet |
|
| 25 |
Maxima Hot Start Green PCR Master Mix (2X) Unit Size 500 x 50 µL rxns Thermo Scientific NAD- K1062 veya muadili |
5 |
Adet |
|
| 26 |
Methanol 2,5 Lt, Merck 106009.2511 veya muadili |
4 |
Adet |
|
| 27 |
One Shot® TOP10 Chemically Competent E. coli, Thermo Scientific, C404006 veya muadili |
2 |
Adet |
|
| 28 |
PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa, 10x250 µL, Thermo Scientific 26620 veya muadili |
4 |
Adet |
|
| 29 |
PCR Tüpü 0,2 ml 8 li strip ve kapağı DNAse Rnase free 120 adt/pkt Steril (120 adetlik paket) |
10 |
Paket |
|
| 30 |
Petri Kutusu (P.S) Gama Steril 90 mm, 500ad/kt (500 adetlik kutu) |
5 |
Kutu |
|
| 31 |
Phosphate buffered saline, Sigma P4417 veya muadili |
2 |
Adet |
|
| 32 |
Precision Plus Protein™ Dual Color Standards, 2.5 ml, Biorad 1610394 veya muadili |
2 |
Adet |
|
| 33 |
PureLink® RNA Mini Kit, Thermo Scientific 12183018A veya muadili |
2 |
Adet |
|
| 34 |
Skim milk powder, 500 g, Sigma 70166-500G veya muadili |
3 |
Adet |
|
| 35 |
Sodium Dodecyl Sulfate (SDS), Lauryl 1 kg, Thermo Scientific 28365 veya muadili |
4 |
Adet |
|
| 36 |
Steril Plastik Öze 100 Ad/pkt (100 adetlik paket) |
10 |
Paket |
|
| 37 |
Storage Box with lid 1,5-2 ml mikrosantrifüj tüpleri için, 100 örn/ad |
10 |
Adet |
|
| 38 |
SuperScript® IV First-Strand Synthesis System, Thermo Scientific 18091200, veya muadili |
2 |
Adet |
|
| 39 |
Supported Nitrocellulose Membrane, Pkg of 1 roll, 0.2 µm, 30 cm x 3 m, Biorad 1620097 veya muadili |
4 |
Adet |
|
| 40 |
Syringe Filters 0,2 µm 50 adet/kutu (50 adetlik kutu) |
5 |
Kutu |
|
| 41 |
Syringe Filters 0,45 µm 50 adet/kutu (50 adetlik kutu) |
5 |
Kutu |
|
| 42 |
TBE 10Xbuffer 1 lt/adet Thermo Scientific # MBT-B52 veya muadili |
4 |
Adet |
|
| 43 |
TC FLASK 25CM2, 160 ad/pkt, steril, DNAse, RNAase free (160 adetlik paket) |
5 |
Paket |
|
| 44 |
TGX™ FastCast™ Acrylamide Kit, 10%, Biorad 1610173 veya muadili |
3 |
Adet |
|
| 45 |
Trans-Blot® Turbo™ Mini PVDF Transfer Packs, Biorad 1704156 veya muadili |
2 |
Adet |
|
| 46 |
Tris Base, 500 g, GE Healtcare 17132101 veya muadili (500 g'lık kutu) |
2 |
Kutu |
|
| 47 |
Tween® 20, 500 ml, Merck 8221840500 veya muadili |
1 |
Adet |
|
| 48 |
10X Tris-Glycine SDS Buffer 1l, Thermo Scientific 28362 veya muadili |
2 |
Adet |
|
| 49 |
2-Mercaptoethanol 250 ml, Merck 8057400250 veya muadili |
2 |
Adet |
|
| 50 |
2x Laemmli Sample Buffer 30 ml, Biorad #1610737 veya muadili |
5 |
Adet |
|
| 51 |
3,3'-Diaminobenzidine tetrahydrochloride, Sigma D5905 veya muadili |
2 |
Adet |
|
| 52 |
4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 50 µl, Biorad 4561094 veya muadili |
4 |
Adet |
|
| 53 |
6X Gel loading Buffer 5x1 ml/adet Thermo Scientific # MBT-R0611 veya muadili |
4 |
Adet |
|
| 54 |
6xHis Protein Tag Stain Reagent Set, 1 l, Thermo Scientific 24570 veya muadili |
4 |
Adet |
|
